Michigan Department of Natural Resources Fisheries Technical Report No. 2002-1, 2002

Methodology for Immersion Marking Walleye Fry and Fingerlings in Oxytetracycline Hydrochloride and Its Detection with Fluorescence Microscopy

David G. Fielder Michigan Department of Natural Resources Alpena Fisheries Research Station 160 East Fletcher Alpena, MI 49707-2344 fielderd@michigan.gov

Abstract.–This manual summarizes the process of conducting walleye stocking evaluations based on immersion marking with oxytetracycline hydrochloride. The summary includes methodology for application of the immersion treatment in the hatchery setting, detection of the mark using fluorescence microscopy and some considerations for planning evaluations based on the technique. The methodology described here was based on available literature and refined to meet the specific needs of the Michigan Department of Natural Resources, Fisheries Division.

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